• Ihre Sprache:
  • Deutsch
  • English

Marie Curie Fellowships

Cultivation and characterisation of co-cultures by using microscaled droplet based platforms - investigation of cell behavior in comparison to established cultivation methods

In the last years miniaturised techniques for cultivation of cells have been established in life sciences. At the Institute for Bioprocessing and Analytical Measurement Techniques (iba) different cultivation techniques basing e.g. on the principle of “Hanging Droplets” were developed. This cultivation technique called “Modified Hanging Droplets” shall be applied to the investigation of cancer cell behaviour depending on different growth conditions and the addition of different drugs. By applying a pipette robot for these investigations high-throughput procedures and protocols shall be developed. These pipette robot based procedures and protocols shall be compared to established protocols to emphasize their advantages. Furthermore, microscopic detection techniques shall be modified and adapted to this cultivation technique.

Development and test of functional modules for the segmented flow based cell cultivation platform “pipe based bioreactors”

In the last years the Institute for Bioprocessing and Analytical Measurement Techniques (iba) has developed different microfluidics based cell cultivation techniques. One of these techniques bases on the principle of segmented flow and has established as “pipe based bioreactors”. The challenges for this platform are to handle cell growth media having high protein content on the one hand and to fulfill the requirements of microfluidics on the other hand. To guarantee essential procedures like droplet generation, medium exchange, droplet and cell detection, and cell harvesting it is necessary to apply special functional modules. Such functional modules have to be developed, to be manufactured (workshop of iba and partners from outside) and to be tested under real cell cultivation conditions at the iba labs.

Proton-proof bilayer structures

Artificial bilayers are workhorse for investigation of ionic channels in a defined manner and with high throughput. Any leakage current should be avoided, making the bilayer a critical element. Using functionalized surfaces and segmented electrodes, we are developing a multichannel sensor on the basis of high quality bilayer structures.  

Ultrafast broad bandwidth bio-impedance measurement

The passive electrical properties of a material are often used for material characterization. Most biological objects show different behavior in different frequency bands where the frequency ranges from mHz up to GHz challenging not only the instrumentation but also algorithms for data mining.

Synchronous multichannel channel impedance spectroscopy

Multichannel impedance measurement systems use generally a sequential approach for reading the behavior of objects attached to a field of electrodes. Dynamic objects with fast change of their passive electrical properties, e.g. after administration of drugs or toxins, undergo already considerable changes during the measurement procedure which makes comparison between the objects attached to different electrodes complicate. We develop a multichannel system with true parallel of the impedance spectrum.

Biomechanics of designer scaffolds made off a photostructurable polymer system by means of Two-Photon-Polymerization (2PP)

Mechanical cues (stiffness, complex modulus) of implantable biomaterials and scaffolds are important for their acceptance in vivo by the host and in vitro by cultured cells. The aim of the project is the development a photostructurable polymer precursor (biophotoresist) with improved biomechanical behavior. Degradable methacrylated short-chain copolymers of D,L-lactide and ε-caprolactone in combination with effective photoinitiating systems are applicable for 3D lithography using femtosecond irradiation. Changes of the relative amounts of the monomers, the molecular weight of the crosslinker and the addition of nanoparticular/nanofibrillar fillers affect the mechanical properties and are adjustable to the biological response of different cell types (bone, cartilage, nerve cells, tumor cells, progenitors). Using nanoindentation and dynamic mechanical analysis under physiological conditions the mechanical properties of these novel microstructured scaffolds (systems properties) can be characterized.

Die Marie Curie Fellowships

Die Marie Curie Fellowships, die im Rahmen des 7. EU-Rahmenprogramms
(FP7 People) vergeben werden, sind darauf ausgerichtet, die Mobilität von jungen Forschenden zu erhöhen. Die Stipendien werden in allen Fachbereichen vergeben.

Europäische Wissenschaftler und Wissenschaftlerinnen können sich für eine Forschungstätigkeit ausserhalb Europas (mit Rückkehrverpflichtung) bewerben (IOF) oder einen Forschungsaufenthalt in einem anderen europäischen Land beantragen (IEF). Forschende, die in Drittstaaten tätig sind, können ein Projekt in Europa durchführen (IIF).

iba e.V. | Rosenhof | 37308 Heilbad Heiligenstadt | +49 (0) 3606 / 671 - 0 | iba(at)iba-heiligenstadt.de | typo3 hannover  microsoft crm add on