Analytical Biophysical Tools To Gain Insights into Interactions between Covid-19 Spike Protein and Blood Cells
- Master thesis for 6 months -
The novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2 or Covid-19), originated in December 2019 is affecting >200 countries, remaining the leading causes of death.
A coronavirus contains spike proteins that recognize angiotensin-converting enzyme 2 (ACE2) on lung cells. A study involving a predominantly minority patient population showed that hospitalized patients with COVID-19 are at risk for developing thrombotic complications, such as VTE, stroke, and limb ischemia.1 However, the role of Covid-19 virus in thrombosis complication is unknown.
Aim of the master thesis:
This project focuses on gaining insights into interactions between Covid-19 spike protein and blood cells such as platelets and endothelial cells using analytical biophysical tools.
Work packages of the master thesis:
- Testing if target plasma proteins enhance binding of Covid-19-spike to blood cells.
- Identification of the role of Covid-19-spike in inducing platelet activation in the presence of several involving plasma proteins.
Methods: Atomic force microscopy (AFM), Quartz Crystal Microbalance (QCM), Surface plasmon resonance (SPR) spectroscopy, Confocal laser scanning microscopy (CLSM), and Flow cytometry.
Profile of qualification and further requirementsStudents of biophysics, chemistry, biology, biochemistry, or biotechnology are welcome to apply. Mater students will be financially supported by iba. The deadline for the application is 31.03.2021.detaillierte Informationen:
Betreuer: Dr. Thi-Huong Nguyen