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Former Junior Research Group

Sensor and actuator systems in biotechnology

Reproducible surface modification plays a key role for sensor surfaces. It determines detection limits and applicability of the sensor. The modification with organosilanes on silicon oxide surfaces is established for producing hydrophilic or hydrophobic surfaces as well as for the immobilization of capture molecules. The functionalized surfaces are applied for the detection of biomolecules such as DNA or proteins and for preventing non-specific adsorption on surfaces [1, 2]. The application of novel surface substrates challenges new modification strategies.

Besides silicon oxide surfaces, waveguide surfaces are of interest for highly sensitive evanescent field sensors [3, 4] and chemical polymers as low-cost substrates [5]. The research focuses as well on the synthesis of photoactive molecules for tailoring surface characteristics and the targeted delivery of surface-bound drugs.
The Junior Research Group addresses both the synthesis of molecules for the required surface properties and the surface modification itself as well as its structuring [6].

Therefore an inert gas working bench and varied surface analysis are available:

  • spectroscopic imaging ellipsometry
  • surface plasmon resonance
  • atomic force microscopy
  • measurement of surface tension / contact angle
  • infrared spectroscopy in attenuated total reflection and transmission
  • scanning electron microscopy
[1] C. Hoffmann, K. Schmitt, A. Brandenburg, S. Hartmann, Rapid protein expression analysis with an interferometric biosensor for monitoring protein production, Anal. Bioanal. Chem. 387 (5/2007) 1921-1932.
[2] C. Hoffmann and G. E. M. Tovar, Mixed self-assembled monolayers (SAMs) consisting of methoxy-tri(ethylene glycol)-terminated and alkyl-terminated dimethylchlorosilanes for controlling the nonspecific adsorption of proteins at oxidic surfaces, J. Colloid Interface Sci., 295 (2006) 427-435.
[3] K. Schmitt, C. Hoffmann, High-refractive-index waveguide platforms for chemical and biosensing, in Springer Series on Chemical Sensors and Biosensors, Vol. 7, Optical Guide-wave Chemical and Biosensors I, Eds.: M. Zourob, A. Lakhtakia, pp 21-54, Springer Verlag Berlin Heidelberg, 2010.
[4] K. Büchner, N. Ehrhardt, Brian P. Cahill, C. Hoffmann, Internal reflection ellipsometry for real-time monitoring of polyelectrolyte multilayer growth onto tantalum pentoxide, Thin Solid Films 519 (2011) 6480-6485.
[5] K. Schmitt, J. Rist, C. Hoffmann, Optical waveguides for evanescent wave induced cleavage of photolabile linker compounds, Anal. Bioanal. Chem. 401 (2/2011) 777-782.
[6] X. Wang, S. Werner, T. Weiß, K. Liefeith, C. Hoffmann, ortho-Nitrobenzyl alcohol based two-photon excitation controlled drug release system, RSC Advances 2 (1/2012) 156-160.
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